DEMO-EM is a hierarchical method to assemble multi-domain protein structures
from cryo-EM density maps.
It starts with query sequence and cryo-EM density maps, with individual domain structure
constructed with
FUpred,
ThreaDom,
and
I-TASSER. Next, the multi-domain complex structure is constructed by a progressive
rigid-body domain assembly and atomic-level flexible structural refinement
process under the guidance of cryo-EM density maps and deep-learning distance profiles.
Large-scale benchmark tests have shown significant advantage of DEMO-EM over
traditional homologous template based approaches for
high-accuracy multi-domain protein structure modeling.
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DEMO-EM Resource:
References:
-
Xiaogen Zhou, Yang Li, Chengxin Zhang, Wei Zheng, Guijun Zhang, Yang Zhang. Progressive assembly of multi-domain protein structures
from cryo-EM density maps. Nature Computational Science, 2: 265-275 (2022).
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