[back]

COFACTOR result for job id 76539

[Download result.tar.bz2 for all prediction results]

User Input

>76539 (295 residues)
MIIQKTYKNNKPTVYLITTPIGNLEDISLRAIQTLKQVDVICCEDTRTSKVLLDKYQITN
NLLSLHKFNENLRIEQIINLLNQNKNIAIISDAGVPIISDPASYIINQLKELEINCNITA
IGAGSAYLHALISSGFLIDNHYFYGFLKNKNKISKQNELNQLINQYGDSIICLYESVHRL
KDTITCLNQLLDKNHKIVIAKELTKINEEIIYGNINQINQYINSEKFVLKGEFVIVINKK
IIDQIINYTDSQLIDLIDQEIKNGYKLKQACEIINLKTKISKNVLYKLYTFKKNF

Download query sequence and structure

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	5hw4A	0.76	0.92	0.311	0.773	Download
2	3kwpA	0.75	1.01	0.360	0.763	Download
3	1wyzB	0.65	2.33	0.187	0.725	Download
4	1wdeA	0.63	3.52	0.118	0.769	Download
5	2dv7A	0.62	3.24	0.116	0.739	Download
6	3i4tA	0.60	3.18	0.137	0.715	Download
7	3nutA	0.58	3.67	0.118	0.725	Download
8	1pjsB	0.57	3.91	0.085	0.749	Download
9	2qbuA	0.56	3.02	0.116	0.675	Download
10	4e16A	0.56	3.86	0.138	0.725	Download

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0008168	1.00	methyltransferase activity
GO:0008757	0.99	S-adenosylmethionine-dependent methyltransferase activity
GO:0008173	0.57	RNA methyltransferase activity
GO:0008649	0.55	rRNA methyltransferase activity
GO:0008171	0.52	O-methyltransferase activity
GO:0070677	0.51	rRNA (cytosine-2'-O-)-methyltransferase activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0071704	1.00	organic substance metabolic process
GO:0044237	1.00	cellular metabolic process
GO:0006807	0.99	nitrogen compound metabolic process
GO:1901360	0.98	organic cyclic compound metabolic process
GO:0046483	0.98	heterocycle metabolic process
GO:0034641	0.98	cellular nitrogen compound metabolic process
GO:0006725	0.98	cellular aromatic compound metabolic process
GO:0044238	0.95	primary metabolic process
GO:0043170	0.93	macromolecule metabolic process
GO:0006139	0.91	nucleobase-containing compound metabolic process
GO:0044260	0.90	cellular macromolecule metabolic process
GO:0090304	0.89	nucleic acid metabolic process
GO:0043412	0.88	macromolecule modification
GO:0016070	0.87	RNA metabolic process
GO:0034660	0.86	ncRNA metabolic process
GO:0034470	0.85	ncRNA processing
GO:0009451	0.84	RNA modification
GO:0006364	0.83	rRNA processing
GO:0001510	0.83	RNA methylation
GO:0031167	0.82	rRNA methylation
GO:0000453	0.81	enzyme-directed rRNA 2'-O-methylation
GO:0009058	0.70	biosynthetic process
GO:1901564	0.69	organonitrogen compound metabolic process
GO:0044249	0.69	cellular biosynthetic process
GO:0018130	0.59	heterocycle biosynthetic process
GO:0051188	0.56	cofactor biosynthetic process
GO:0044763	0.55	single-organism cellular process
GO:0044711	0.55	single-organism biosynthetic process
GO:0033013	0.55	tetrapyrrole metabolic process
GO:0044281	0.53	small molecule metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0005737	1.00	cytoplasm
GO:0044444	0.89	cytoplasmic part
GO:0005829	0.68	cytosol

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.082	1vhvB	0.611	2.88	0.123	0.715	2.1.1.98	26,28,94,125
2	0.067	1wdeA	0.633	3.52	0.118	0.769	2.1.1.98	16,93,173
3	0.067	1v9aB	0.539	3.93	0.098	0.702	2.1.1.107	NA
4	0.067	1ve2A	0.546	3.42	0.126	0.678	2.1.1.107	45
5	0.067	2e0kB	0.553	3.77	0.127	0.709	2.1.1.130	27,122,233

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
	1	0.68	2hutA	0.617	3.25	0.122	0.739	0.75	SAH	Download	21,94,95,98,125,126,174,175,202,203,232,233

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Download result.tar.bz2 for all prediction results]

Reference:

Chengxin Zhang, Peter L Freddolino, and Yang Zhang. COFACTOR: Improved protein function prediction by combining structure, sequence, and protein-protein interaction information. Nucleic Acids Research, 45: W291-299 (2017)
Ambrish Roy, Jianyi Yang, and Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, 40:W471-W477 (2012).

[back]