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COFACTOR result for job id 76293

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>76293 (855 residues)
MQSKISWTLSKKSKYLFGSDTTKTKLKYIKGSIFSIIVGFIVSGIFLSFLNINPFTYFAL
LFGINFDKNFYQISLNWMAVYIVAGLSMAIAFKSGVFNIGASGQILTATSVATIVFFSIS
GKDASSITPFMIILMLITCIISAAFIAFIAGILKALFNIHEVVSTILLNWSVFYIFKWFF
GRYQDFSSGLSYTSKNIPSDQLSIGSNTVIIPLLIALICVIIIWILFSKTVLGFKLKAVG
SSITGSKYIGINVKRQIITSLTLSGAVAGIAGFLSMFTVSPNNFFASNSLPTLGFDAIAV
SLVAFNNPIGIIAIGWLWAIIKTGGGPISSLYSISTQISGLISGILIYFTAIVSVFIAFK
PWELLKNKYNLYTSKVNREIYWKLKLYTFKLRLKKIFLIFTKDYKQEVNNKYQIYTKQNQ
ITNKTSNPHLFWNGRKNIEIELKNDLKQSIYLVKSKIDEIKKFVDQDKNSLNVSGLKNDL
NKQVNLLASKYIQNLRDLDLELADHKYKIQKITSSILNEYQTNIKQAKKTHRLKIQQIKV
FKESQIGIITYKFDSHNNIIEIKANKLKTIAQLKEQIKNIKSELRLEKQISNLNKSSTQT
NNSEKLEKIKQLKEQIKVVRKQANAKILEQKNKYKNQKNSVKQEQVQLQDILHQYNNYLN
KEKDRFKESKKAALVLKQKRLQAIDMNLSQSDVNKAVSLLNDLKTLITDNLDLNLNKQAI
KSNQKTLKNALEIKSKIDEVLTQNIISEYDAPEHIKQKSKISLTTFKLITNLKKQISYVI
TRVEEQELIDKYQQWISQAKQVVQDEKNNYEQIIKKAPRKNLADLENLFNLEKSLKEQTN
LKILNLTNTMLKETK

Download query sequence and structure

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	5u1sA	0.30	8.20	0.039	0.462	Download
2	5hb4B	0.30	8.39	0.047	0.450	Download
3	1xi5A	0.29	8.41	0.030	0.454	Download
4	3jcrA	0.29	8.50	0.039	0.447	Download
5	6hydA	0.26	9.11	0.057	0.423	Download
6	5a9qA	0.25	8.37	0.051	0.390	Download
7	4w8jA	0.25	8.20	0.055	0.381	Download
8	6edoA	0.25	8.65	0.034	0.395	Download
9	5yz0A	0.25	8.21	0.040	0.378	Download
10	4rcnA	0.25	8.35	0.030	0.384	Download

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	0.89	catalytic activity
GO:0016787	0.58	hydrolase activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.92	cellular process
GO:0008152	0.70	metabolic process
GO:0071704	0.69	organic substance metabolic process
GO:0044237	0.69	cellular metabolic process
GO:0044699	0.66	single-organism process
GO:0044238	0.66	primary metabolic process
GO:0006807	0.64	nitrogen compound metabolic process
GO:1901360	0.60	organic cyclic compound metabolic process
GO:0034641	0.60	cellular nitrogen compound metabolic process
GO:0046483	0.59	heterocycle metabolic process
GO:0051179	0.58	localization
GO:0044763	0.58	single-organism cellular process
GO:0006725	0.57	cellular aromatic compound metabolic process
GO:0051234	0.56	establishment of localization
GO:0006139	0.56	nucleobase-containing compound metabolic process
GO:0006810	0.52	transport
GO:0016043	0.50	cellular component organization

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0044446	0.95	intracellular organelle part
GO:0032991	0.93	macromolecular complex
GO:0044444	0.70	cytoplasmic part
GO:0043226	0.69	organelle
GO:0043234	0.68	protein complex
GO:0043229	0.67	intracellular organelle
GO:0016020	0.63	membrane
GO:0043227	0.61	membrane-bounded organelle
GO:0044428	0.59	nuclear part
GO:0043231	0.57	intracellular membrane-bounded organelle
GO:0044430	0.52	cytoskeletal part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2vkzG	0.227	9.27	0.035	0.379	2.3.1.38 3.1.2.14	NA
2	0.060	2pflA	0.190	7.89	0.045	0.283	2.3.1.54	NA
3	0.060	3ixzA	0.211	7.62	0.026	0.304	3.6.3.10	NA
4	0.060	2qtcB	0.190	8.09	0.050	0.286	1.2.4.1	NA
5	0.060	1wz2A	0.191	9.60	0.030	0.333	6.1.1.4	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
	1	0.01	1utcA	0.118	6.82	0.034	0.164	0.16	III	Download	39,40,313
	2	0.01	2uvcG	0.227	8.68	0.022	0.359	0.10	NAP	Download	41,313,314

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Download result.tar.bz2 for all prediction results]

Reference:

Chengxin Zhang, Peter L Freddolino, and Yang Zhang. COFACTOR: Improved protein function prediction by combining structure, sequence, and protein-protein interaction information. Nucleic Acids Research, 45: W291-299 (2017)
Ambrish Roy, Jianyi Yang, and Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, 40:W471-W477 (2012).

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