• Specify secondary structure
    If you have some knowledge on the secondary structure for some residues of your target sequence, you can use this option to upload a text file to specify their secondary structure. I-TASSER will try to generate its models following the secondary structure that you specified.

    Each row in the text file should follow the format:
    "Res_#   Res_Name   Secondary_Structure",

    1st column: residue number,
    2nd column: residue name,
    3rd column: secondary structure (S: Strand, H: Helix, C: Coil).

    The columns should be seperated by white space.

    Below is an example: 

    21    V   S
22    P   S
23    K   S
24    E   S
25    Q   S
26    R   S
27    V   S
28    D   C
29    S   C
30    L   C
31    I   C
125   A   S
126   E   S
127   H   S
128   F   S
129   Y   S
130   W   S
131   M   S
181   V   H
182   S   H
183   C   H
184   L   H
185   K   H
    Download the text file for the above example.